Head of Lab: Prof. Ludomira Granicka, PhD, DSc


Monika Drabik, PhD Eng.
Anna Grzeczkowicz, PhD
Angelika Kwiatkowska, PhD Eng.
Agata Lipko, PhD
Witold Średnicki, MSc

Topic: Nanobiosystems for biological processes regulation for therapeutic purposes

(Research area: nanobiomaterial engineering, nanohybrid systems for regulating biological processes)

The Laboratory uses and develops modern nanobiomaterial engineering techniques in systems for regulating biological processes to support or replace the functions of tissues or organs or for anti-cancer activity.
Work is carried out in the following aspects:
a) construction of targeted systems for delivering therapeutic or anti-cancer agents;
b) design of systems built of nanoelements with antimicrobial activity to cooperate with eukaryotic cells;
c) application of nanobiomaterials in systems for supporting the directed growth of eukaryotic cells.

This involves, among others, the preparation and characterization of materials that demonstrate high application potential in terms of their later use in nanosystems intended, among others, for releasing a therapeutic agent. Within the characterization of biomaterials we standardized methods of biocompatibility assessment.

In addition, work is carried out using the immobilization of biologically active material in membranes. Among the techniques we use, we should mention bioencapsulation, including nanoencapsulation, macroencapsulation and microencapsulation.

The research programs carried out in the Laboratory concerned, among others, effective nanocoating of biological material for immunoisolation purposes, including artificial pancreas, as well as analysis of the influence of factors produced by immobilized microorganisms on eukaryotic cells. Recently, work has been carried out on the construction of multilayer nanocomposite systems used to support the healing of difficult-to-heal wounds.

The conducted research uses, among others: confocal microscopy, atomic force microscopy, spectroscopy, scanning electron microscopy, as well as flow cytometry and fluorescence microscopy.

Keywords: bioencapsulation, immobilization, membrane, metallic nanoparticles, nanosystem, biocompatibility, anticancer therapy, antibacterial activity, antiviral activity, healing of difficult-to-heal wounds, nanobiomaterial.

Selected publications from the JCR list from the last 10 years

1. Kwiatkowska A., Wierzchowski K., Lipko A., Grzeczkowicz A., Antosiak-Iwańska M., Drabik M., Strawski M., Nowak B., Pilarek M., Granicka L.H.: Albumin-derived perfluorodecalin-based hybrid systems with developed effective surfaces aimed at biomedical application. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024;694:134157. doi:10.1016/j.colsurfa.2024.134157.
2. Miłek, T.; Grzeczkowicz, A.; Lipko, A.; Mesjasz, M.; Oklesiński, L.; Kwiatkowska, A.; Strawski, M.; Drabik, M.; Granicka, L.H. An Oxygenated Layer Coating to Enhance the Healing Process of Non-Healing Wounds—A Case Report. Appl. Sci. 2024, 14, 11200. doi.org/10.3390/app142311200
3. Lipko, A.; Grzeczkowicz, A.; Antosiak-Iwańska, M.; Strawski, M.; Drabik, M.; Kwiatkowska, A.; Godlewska, E.; Granicka, L.H.: Polyelectrolyte Platforms with Copper Nanoparticles as a Multifunctional System Aimed at Healing Process Support. Processes, 2024;12:512. doi: 10.3390/pr12030512.
4. Kwiatkowska A., Granicka LH. Anti-Viral Surfaces in the Fight against the Spread of Coronaviruses. Membranes, 2023;13:464. doi: 10.3390/membranes13050464.
5. Miłek, T.; Grzeczkowicz, A.; Lipko, A.; Oleksinski, L.; Kwiatkowska, A.; Strawski, M.; Drabik, M.; Stachowiak, R.; Goliszewski, J.; Granicka, L.H. A Functionalized Membrane Layer as Part of a Dressing to Aid Wound Healing. Membranes, 2022;12:936. doi: 10.3390/ membranes12100936.
6. Grzeczkowicz, A.; Lipko, A.; Kwiatkowska, A.; Strawski, M.; Bącal, P.; Więckowska, A.; Granicka, L.H. Polyelectrolyte Membrane Nanocoatings Aimed at Personal Protective and Medical Equipment Surfaces to Reduce Coronavirus Spreading. Membranes, 2022;12:946. doi: 10.3390/ membranes12100946.
7. A. Kwiatkowska, M. Drabik, A. Lipko, A. Grzeczkowicz, R. Stachowiak, A. Marszalik, L.H. Granicka. Composite Membrane Dressings System with Metallic Nanoparticles as an Antibacterial Factor in Wound Healing; Membranes (Basel), 2022;12(2):215 doi: 10.3390/membranes12020215
8. A. Grzeczkowicz, A. Kwiatkowska, M. Drabik, L. Granicka: Nanoencapsulation of neuronal cells for cryopreservation purposes. Desalination and Water Treatment, 2021; 1-11; doi: 10.5004/dwt.2021.26655.
9. M. Drabik, A. Grzeczkowicz, P. Bącal, A. Kwiatkowska, M. Strawski, M. Antosiak-Iwańska, B. Kazimierczak, Ewa Godlewska and Ludomira H. Granicka: Nanocomposite Membrane Scaffolds for Cell Function Maintaining for Biomedical Purposes. Nanomaterials, 2021, 11, 1094. https://doi.org/10.3390/nano11051094.
10. A. Grzeczkowicz, M. Drabik, A. Lipko, P. Bącal, A. Kwiatkowska, Kazimierczak B., and L.H. Granicka: A Composite Membrane System with Gold Nanoparticles, Hydroxyapatite, and Fullerenol for Dual Interaction for Biomedical Purposes. Membranes, 2021; 11(8): 565. https://doi.org/10.3390/membranes11080565.
11. Drabik, M.; Grzeczkowicz, A.; Bącal, P.; Kwiatkowska, A.;Strawski, M.; Antosiak-Iwańska, M.;Kazimierczak, B.; Godlewska, E.;Granicka, L.H. Nanocomposite Membrane Scaffolds for Cell Function Maintaining for Biomedical Purposes. Nanomaterials, 2021; 11,1094. https://doi.org/10.3390/nano11051094
12. A. Grzeczkowicz, A. Kwiatkowska, M. Drabik, L. Granicka Nanoencapsulation of neuronal cells for cryopreservation purposes. Desalination and Water Treatment, 2021; 214:135–145
13. M. Antosiak-Iwańska, P. Bącal, B. Kazimierczak, A. Kwiatkowska, E. Godlewska, A. Grzeczkowicz, R. Stachowiak, J. Bielecki, L.H. Granicka. Polyelectrolyte Membrane with Hydroxyapatite and Silver Nanoparticles as a Material for Modern Wound Dressings. J Biomed Nanotechnol. 2020;16(5):702-714. doi: 10.1166/jbn.2020.2907.
14. A. Trusek, E. Kijak, L. Granicka: Graphene oxide as a potential drug carrier – chemical carrier activation, drug attachment and its enzymatic release. Materials Science & Engineering C, 2020;116:111240; https://doi.org/ 10.1016/j.msec.2020.111240.
15. Dybowska-Sarapuk L., Weronika Sosnowicz, Jakub Krzeminski, Anna Grzeczkowicz, Ludomira H. Granicka, Andrzej Kotela and Malgorzata Jakubowska: Printed Graphene Layer as a Base for Cell Electrostimulation—Preliminary Results. Int. J. Mol. Sci., 2020; 21(21), 7865; doi:10.3390/ijms21217865.
16. A. Grzeczkowicz, J. Gruszczyńska-Biegala, M. Czeredys, A. Kwiatkowska, M. Strawski, M. Szklarczyk, M. Koźbiał, J. Kuźnicki, L. H. Granicka: Polyelectrolyte membrane scaffold sustains growth of neuronal cells. J Biomed Mater Res Part A, 2019; 107(4):839-850.
17. A. Kwiatkowska, L. H. Granicka, A. Grzeczkowicz, R. Stachowiak, P. Bącal, K. Sobczak, M. Darowski, M. Kozarski, J. Bielecki: Gold nanoparticle-modified poly(vinyl chloride) surface with improved antimicrobial properties for medical devices. J. Biomed. Nanotechnol. 2018;14: 922-932.
18. A. Kwiatkowska, L. H. Granicka, A. Grzeczkowicz, R. Stachowiak, M. Kamiński, Z. Grubek, J. Bielecki, M. Strawski, M. Szklarczyk: Stabilized nanosystem of nanocarriers with an immobilized biological factor for anti-tumor therapy. PlosOne, 2017; doi. 10.1371/ journal.pone.0170925.
19. Marcin Strawski, Ludomira H. Granicka, Marek Szklarczyk: Redox properties of polyelectrolyte multilayer modified electrodes: a significant effect of the interactions between the polyelectrolyte layers in the films. Electrochimica Acta, 2017; 226: 121–131.
20. A. Kwiatkowska, L. Granicka, A. Grzeczkowicz, A. Chwojnowski, C. Wojciechowski, Nano-thin polielectrolytes layers for biotechnological applications. Desalination and Water Treatment, 2017; 64: 260-265.
21. A. Grzeczkowicz, L. H. Granicka, I. Maciejewska, M. Strawski, M. Szklarczyk, M. Borkowska: The experimental study of the performance of nano-thin polyelectrolyte shell for dental pulp stem cells immobilization. J. Nanosci. Nanotechnol. 2015;15, 9531-9538.
22. Alicja Mackiewicz, Anna Grzeczkowicz, Ludomira Granicka, Magdalena Antosiak-Iwańska, Ewa Godlewska, Dariusz Gozdowski, Dorota Olczak-Kowalczyk: Cytotoksyczność materiału Nanocare Gold® w badaniu in vitro – badanie pilotażowe (Cytotoxicity of Nanocare Gold® in In Vitro Assay – Pilot Study). Dent. Med. Probl. 2015; 52, 2, 167–174.
23. M. Borkowska, A. Grzeczkowicz, M. Strawski, J. Kawiak, M. Szklarczyk, L. H. Granicka: Performance and detection of nano-thin polyelectrolyte shell for cell coating. J Nanopart Res, 2014; 16 (7): art. no. 2488.
24. Ludomira H. Granicka: Nanoencapsulation of Cells Within Multilayer Shells for Biomedical Applications. J Nanosci Nanotechnol, 2014;14, 705–716.
25. L. H. Granicka, M. Borkowska, A. Grzeczkowicz, R. Stachowiak, M. Szklarczyk, J. Bielecki, M. Strawski: The targeting nanothin polyelectrolyte shells in system with immobilized bacterial cells for antitumor factor production. J Biomed Mater Res A, 2014; 102 (8): 2662-2668.

Patents

1. L. Granicka, A. Grzeczkowicz, A. Kwiatkowska: Układ membranowy do miejscowej ukierunkowanej immobilizacji komórek oraz sposób jego wytwarzania, Patent nr 236035; 2020.
2. L. Granicka, A. Grzeczkowicz, M. Borkowska: Układ membranowy do miejscowej immobilizacji komórek oraz sposób jego wytwarzania. Patent nr P-403498; 2016.
3. L. Granicka, J. Kawiak, R. Stachowiak, J. Wiśniewski, J. Bielecki, M. Łyżniak: "Membrana poliolefinowa do izolacji mikroorganizmów Gram +". Patent nr P-389847; 2012.